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mouse testis marathon readytm double stranded cdna library  (TaKaRa)


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    TaKaRa mouse testis marathon readytm double stranded cdna library
    FIG. 2. Expression of PMCA4 in mouse testis and in sperm. A, Northern blot demonstrating expression of PMCA4 in mouse testis. Approximately 50 pg of PMCA4 <t>cDNA</t> was spotted onto the membrane as positive control (pos. control). Loading control: -actin. Sk. Muscle, skeletal muscle. B–E, localization of PMCA4b to the principal piece of the tail and acrosome region of mouse sperm as follows. B, phase contrast micrograph of wild-type sperm. C, staining of PMCA4 in the same sperm with polyclonal PMCA4-specific antibodies and CyTM3- labeled secondary antibody. D, phase contrast of control staining of wild-type sperm. E, control staining with secondary antibody alone, showing no unspecific staining.
    Mouse Testis Marathon Readytm Double Stranded Cdna Library, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+testis+marathon+readytm+double/Mouse+Testis+Marathon+-Ready+cDNA/10__1074_slash_jbc__m312599200-37-17-26
    Average 93 stars, based on 90 article reviews
    mouse testis marathon readytm double stranded cdna library - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Plasma Membrane Ca2+ ATPase 4 Is Required for Sperm Motility and Male Fertility"

    Article Title: Plasma Membrane Ca2+ ATPase 4 Is Required for Sperm Motility and Male Fertility

    Journal: Journal of Biological Chemistry

    doi: 10.1074/jbc.m312599200

    FIG. 2. Expression of PMCA4 in mouse testis and in sperm. A, Northern blot demonstrating expression of PMCA4 in mouse testis. Approximately 50 pg of PMCA4 cDNA was spotted onto the membrane as positive control (pos. control). Loading control: -actin. Sk. Muscle, skeletal muscle. B–E, localization of PMCA4b to the principal piece of the tail and acrosome region of mouse sperm as follows. B, phase contrast micrograph of wild-type sperm. C, staining of PMCA4 in the same sperm with polyclonal PMCA4-specific antibodies and CyTM3- labeled secondary antibody. D, phase contrast of control staining of wild-type sperm. E, control staining with secondary antibody alone, showing no unspecific staining.
    Figure Legend Snippet: FIG. 2. Expression of PMCA4 in mouse testis and in sperm. A, Northern blot demonstrating expression of PMCA4 in mouse testis. Approximately 50 pg of PMCA4 cDNA was spotted onto the membrane as positive control (pos. control). Loading control: -actin. Sk. Muscle, skeletal muscle. B–E, localization of PMCA4b to the principal piece of the tail and acrosome region of mouse sperm as follows. B, phase contrast micrograph of wild-type sperm. C, staining of PMCA4 in the same sperm with polyclonal PMCA4-specific antibodies and CyTM3- labeled secondary antibody. D, phase contrast of control staining of wild-type sperm. E, control staining with secondary antibody alone, showing no unspecific staining.

    Techniques Used: Expressing, Northern Blot, Membrane, Positive Control, Control, Staining, Labeling

    Related Articles

    Cloning:

    Article Title: Plasma Membrane Ca2+ ATPase 4 Is Required for Sperm Motility and Male Fertility
    Article Snippet: .. Cloning of Mouse PMCA4 Isoforms—Mouse PMCA4 isoforms were cloned by rapid amplification of cDNA ends from a mouse testis Marathon-ReadyTM double-stranded cDNA library (catalog number 7455-1, Clontech) using the Advantage® 2 PCR system (Clontech) and the following gene-specific primers: mouse PMCA4 forward, 5 -GTC TGA TCA TGT CTG TCC TCA CAG TTG-3 , and mouse PMCA4 reverse, 5 -GCA GCC CCT CTG GCA CAG CCA CT-3 . .. Polymerase chain reaction was performed as suggested by the manufacturer, and the resulting PCR fragments were cloned into pCR®-XL-TOPO vector (Invitrogen) and subsequently sequenced with the standard T7 and M13 reverse primers.

    Clone Assay:

    Article Title: Plasma Membrane Ca2+ ATPase 4 Is Required for Sperm Motility and Male Fertility
    Article Snippet: .. Cloning of Mouse PMCA4 Isoforms—Mouse PMCA4 isoforms were cloned by rapid amplification of cDNA ends from a mouse testis Marathon-ReadyTM double-stranded cDNA library (catalog number 7455-1, Clontech) using the Advantage® 2 PCR system (Clontech) and the following gene-specific primers: mouse PMCA4 forward, 5 -GTC TGA TCA TGT CTG TCC TCA CAG TTG-3 , and mouse PMCA4 reverse, 5 -GCA GCC CCT CTG GCA CAG CCA CT-3 . .. Polymerase chain reaction was performed as suggested by the manufacturer, and the resulting PCR fragments were cloned into pCR®-XL-TOPO vector (Invitrogen) and subsequently sequenced with the standard T7 and M13 reverse primers.

    Rapid Amplification of cDNA Ends:

    Article Title: Plasma Membrane Ca2+ ATPase 4 Is Required for Sperm Motility and Male Fertility
    Article Snippet: .. Cloning of Mouse PMCA4 Isoforms—Mouse PMCA4 isoforms were cloned by rapid amplification of cDNA ends from a mouse testis Marathon-ReadyTM double-stranded cDNA library (catalog number 7455-1, Clontech) using the Advantage® 2 PCR system (Clontech) and the following gene-specific primers: mouse PMCA4 forward, 5 -GTC TGA TCA TGT CTG TCC TCA CAG TTG-3 , and mouse PMCA4 reverse, 5 -GCA GCC CCT CTG GCA CAG CCA CT-3 . .. Polymerase chain reaction was performed as suggested by the manufacturer, and the resulting PCR fragments were cloned into pCR®-XL-TOPO vector (Invitrogen) and subsequently sequenced with the standard T7 and M13 reverse primers.

    cDNA Library Assay:

    Article Title: Plasma Membrane Ca2+ ATPase 4 Is Required for Sperm Motility and Male Fertility
    Article Snippet: .. Cloning of Mouse PMCA4 Isoforms—Mouse PMCA4 isoforms were cloned by rapid amplification of cDNA ends from a mouse testis Marathon-ReadyTM double-stranded cDNA library (catalog number 7455-1, Clontech) using the Advantage® 2 PCR system (Clontech) and the following gene-specific primers: mouse PMCA4 forward, 5 -GTC TGA TCA TGT CTG TCC TCA CAG TTG-3 , and mouse PMCA4 reverse, 5 -GCA GCC CCT CTG GCA CAG CCA CT-3 . .. Polymerase chain reaction was performed as suggested by the manufacturer, and the resulting PCR fragments were cloned into pCR®-XL-TOPO vector (Invitrogen) and subsequently sequenced with the standard T7 and M13 reverse primers.

    Polymerase Chain Reaction:

    Article Title: Plasma Membrane Ca2+ ATPase 4 Is Required for Sperm Motility and Male Fertility
    Article Snippet: .. Cloning of Mouse PMCA4 Isoforms—Mouse PMCA4 isoforms were cloned by rapid amplification of cDNA ends from a mouse testis Marathon-ReadyTM double-stranded cDNA library (catalog number 7455-1, Clontech) using the Advantage® 2 PCR system (Clontech) and the following gene-specific primers: mouse PMCA4 forward, 5 -GTC TGA TCA TGT CTG TCC TCA CAG TTG-3 , and mouse PMCA4 reverse, 5 -GCA GCC CCT CTG GCA CAG CCA CT-3 . .. Polymerase chain reaction was performed as suggested by the manufacturer, and the resulting PCR fragments were cloned into pCR®-XL-TOPO vector (Invitrogen) and subsequently sequenced with the standard T7 and M13 reverse primers.



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    mouse testis marathon readytm double stranded cdna library  (TaKaRa)
    93
    TaKaRa mouse testis marathon readytm double stranded cdna library
    FIG. 2. Expression of PMCA4 in mouse testis and in sperm. A, Northern blot demonstrating expression of PMCA4 in mouse testis. Approximately 50 pg of PMCA4 <t>cDNA</t> was spotted onto the membrane as positive control (pos. control). Loading control: -actin. Sk. Muscle, skeletal muscle. B–E, localization of PMCA4b to the principal piece of the tail and acrosome region of mouse sperm as follows. B, phase contrast micrograph of wild-type sperm. C, staining of PMCA4 in the same sperm with polyclonal PMCA4-specific antibodies and CyTM3- labeled secondary antibody. D, phase contrast of control staining of wild-type sperm. E, control staining with secondary antibody alone, showing no unspecific staining.
    Mouse Testis Marathon Readytm Double Stranded Cdna Library, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+testis+marathon+readytm+double/Mouse+Testis+Marathon+-Ready+cDNA/10__1074_slash_jbc__m312599200-37-17-26
    Average 93 stars, based on 1 article reviews
    mouse testis marathon readytm double stranded cdna library - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    FIG. 2. Expression of PMCA4 in mouse testis and in sperm. A, Northern blot demonstrating expression of PMCA4 in mouse testis. Approximately 50 pg of PMCA4 cDNA was spotted onto the membrane as positive control (pos. control). Loading control: -actin. Sk. Muscle, skeletal muscle. B–E, localization of PMCA4b to the principal piece of the tail and acrosome region of mouse sperm as follows. B, phase contrast micrograph of wild-type sperm. C, staining of PMCA4 in the same sperm with polyclonal PMCA4-specific antibodies and CyTM3- labeled secondary antibody. D, phase contrast of control staining of wild-type sperm. E, control staining with secondary antibody alone, showing no unspecific staining.

    Journal: Journal of Biological Chemistry

    Article Title: Plasma Membrane Ca2+ ATPase 4 Is Required for Sperm Motility and Male Fertility

    doi: 10.1074/jbc.m312599200

    Figure Lengend Snippet: FIG. 2. Expression of PMCA4 in mouse testis and in sperm. A, Northern blot demonstrating expression of PMCA4 in mouse testis. Approximately 50 pg of PMCA4 cDNA was spotted onto the membrane as positive control (pos. control). Loading control: -actin. Sk. Muscle, skeletal muscle. B–E, localization of PMCA4b to the principal piece of the tail and acrosome region of mouse sperm as follows. B, phase contrast micrograph of wild-type sperm. C, staining of PMCA4 in the same sperm with polyclonal PMCA4-specific antibodies and CyTM3- labeled secondary antibody. D, phase contrast of control staining of wild-type sperm. E, control staining with secondary antibody alone, showing no unspecific staining.

    Article Snippet: Cloning of Mouse PMCA4 Isoforms—Mouse PMCA4 isoforms were cloned by rapid amplification of cDNA ends from a mouse testis Marathon-ReadyTM double-stranded cDNA library (catalog number 7455-1, Clontech) using the Advantage® 2 PCR system (Clontech) and the following gene-specific primers: mouse PMCA4 forward, 5 -GTC TGA TCA TGT CTG TCC TCA CAG TTG-3 , and mouse PMCA4 reverse, 5 -GCA GCC CCT CTG GCA CAG CCA CT-3 .

    Techniques: Expressing, Northern Blot, Membrane, Positive Control, Control, Staining, Labeling